attune flow cytometer - An Overview
attune flow cytometer - An Overview
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Info-enriched characterization of mobile populations. Morphological info from photographs can insert towards the richness of flow cytometry facts. As an example, the determine shows an in any other case traditional apoptosis assay working with Annexin V and PI, adding mobile imaging to characterize cells in Every single populace to reveal morphologically distinct capabilities. These insights could not have already been attained from multiplex staining by yourself.
No matter if you’re a beginner or an expert, discover our resources to progress your study and know-how in flow cytometry.
Incorporate acoustic concentrating technology with superior-parameter Investigation for flexible spectral unmixing and conventional workflows.
The award-winning Invitrogen Attune CytPix Flow Cytometer combines acoustic concentrating which has a large-speed brightfield digital camera for simultaneous higher throughput flow cytometry and significant resolution brightfield imaging.
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In this article, a skilled consumer has gated singlets confidently. After analyzing the manual singlet gate, the CytPix impression-derived parameter ParticleCount reveals this gate consists of in excess of 4% aggregates.
Optimizing gating strategy. Even sturdy manual singlet gating is error-susceptible and stays a subjective determination level in Just about all flow cytometry assays. Imaging may be used to confirm and change gates to incorporate only one cells of interest.
Dependable picture high-quality even at significant throughput Acoustic concentrating along with a higher-speed digicam Merge to image these T cells continually at reduced or higher flow charges. Conveniently alter aim and camera settings to meet experimental prerequisites.
The Attune NxT cytometer could be the reliable workhorse instrument your lab can rely upon for reliable and dependable functionality. The Attune CytPix product incorporates a large-speed brightfield digital camera and AI-pushed graphic Evaluation software program, with the latest upgrades like device Discovering instruments and an intuitive user interface, enabling Improved facts precision, precision, and more rapidly insights into sample biology.
Users can perspective party visuals in authentic-time to substantiate sample high quality, optimize protocols, and record the morphology of each and every cell population identified for analysis. Imaging capture also Rewards from acoustic concentrating, which helps to situation the cells making sure that a pointy, centered picture is obtained.
AI-pushed automatic impression Assessment While using the Attune Cytometric Software package interprets occasion features into distinctive morphology parameters which can be combined with common fluorescence and scatter parameters to expose remarkably reproducible populace improvements and one of a kind subsets.
We Earlier shown the power of imaging Vehicle-T/Ramos mobile interactions. Let’s take a look at just the inhabitants of greatest interest, the double favourable activities, to learn more. We can easily now use prolonged morphological parameters (in this article, circularity and skewness of intensity) to even more look at the characteristics of those populations and refine gating on these situations, growing knowledge robustness.
Constructed-in dimensionality reduction instruments improved resolution into populations. Fluorescently gated populations back-gated on to UMAP plot to verify expected populace resolution in lysed whole human blood. User can see events from Every cluster for visual affirmation of cell phenotypes.
Adaptable payment approach from Agilent Money Answers allow you to have the important instrumentation you would like for rapid job, or to increase your lab.
Gating on these extended parameters means that you can speedily and properly recognize populations of curiosity and confirm gating tactic with little or no manual overview. The Attune program gives usage of condition-of-the-art equipment Finding out Assessment applications in just an expertly built consumer interface, rendering it exceptionally easy to use.
Since the cell line had Earlier been analyzed over the Attune CytPix Flow Cytometer, the scientists went again to the photographs and ended up in the position to document the microbial infection at least 5 times previously.
Agilent Value Assure assures you a minimum of ten years of instrument use from the day you buy. Otherwise, We're going to credit rating you Together with the residual price of the program toward an upgraded read more design.
What appeared to be a homogeneous population is really extra various—an insight that ought to be considered when interpreting effects.